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| sodium orthovanadate ： ウィキペディア英語版 |
sodium orthovanadate
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Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43−. It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO43− ion binds reversibly to the active sites of most protein tyrosine phosphatases.
It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final working concentration of 1 - 10 mM. It is toxic when inhaled, swallowed or in contact with skin.
==Synthesis==
Sodium orthovanadate is created by dissolving vanadium(V) oxide in a solution of sodium hydroxide.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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